Background: Cell cycle progression is subject to arrest at G1 and G2 checkpoints in response to DNA damage, presumably to allow time for DNA repair prior to entry into S and M phase, respectively. The p53 tumor suppressor is required for one such G1 checkpoint and functions to upregulate expression of GADD 45 and p21. GADD 45 binds both Cdks and PCNA, a protein involved in DNA replication and repair. GADD 45 stimulates DNA excision repair in vitro and in hibits entry of cells into S phase. Thus, it has been suggested that GADD 45 may serve as a link between the p53-dependent cell cycle checkpoint and DNA repair. GADD 45-like proteins, GADD 45β and GADD 45γ, have been shown to be induced by environmental stresses. GADD 45β and GADD 45γ, are thought to induce p38/JNK activation via MEKK4 activation.
Description: Rabbit polyclonal to GADD45G
Immunogen: KLH conjugated synthetic peptide derived from GADD45G
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 17 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
