Background: The death-associated protein 3 (DAP-3), is a nucleotide-binding protein that contains a potential P-loop motif. It is a positive mediator of programmed cell death; overexpressed intact full-length protein is required in order to induce apoptosis. DAP-3 functions downstream of its receptor signaling complex and its death promoting effects depend on caspase activity. It also interacts with the glucocorticoid receptor (GR), where its main interaction domain is the amino-terminal region, which acts in a dominant-negative fashion to protect cells from apoptosis. DAP-3 protein may also play a role in modulating the cytoplasmic GR/HSP 90 complex. It is conserved at the functional, as well as the structural level, and is ubiquitously expressed in highly proliferative epithelial compartments of various tissues. Unlike a number of other proteins, DAP-3 retains its mitochondrial localization during the induction of apoptosis.
Description: Rabbit polyclonal to DAP3
Immunogen: KLH conjugated synthetic peptide derived from DAP3
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 45 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
