Background: Nox1 and the glycoprotein gp91 phox are largely related proteins that are essential components of the NADPH oxidase. The superoxide-generating NADPH oxidase is present in phagocytes, neuroepithelial bodies, vascular smooth muscle cells, and endothelial cells. It includes a membrane-bound flavocytochrome containing two subunits, gp91 phox and p22 phox, and the cytosolic proteins p47 phox and p67 phox. During activation of the NADPH oxidase, p47 phox and p67 phox migrate to the plasma membrane where they associate with the flavocytochrome, cytochrome b558, to form the active enzyme complex. The p22 and gp91 phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. Nox1 and gp91 contain identical C-terminal sequence identity, yet they have distinct expression patterns. gp91 phox is expressed in eosinophils, neutrophils, monocytes, and B-lymphocytes, whereas Nox1 is predominantly detected in the colon, and low expression is also detected in the uterus and prostate. Nox1 is also upregulated in vascular smooth-muscle cells in response to PDGF stimulation, which collectively indicates that Nox1 may function analogously to gp91 phox, yet regulate the NADPH superoxide production in non-phagocytic cells.
Description: Rabbit polyclonal to NOX1
Immunogen: KLH conjugated synthetic peptide derived from NOX1
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 65 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
